What Are The Disinfection Methods For Bioreactors?

2023-10-12 15:15:42
What Are The Disinfection Methods For Bioreactors?
After completing the cleaning of the fermentation system, the next step is to empty the fermentation tank (empty tank disinfection).


The definition of air disinfection: to remove bad or unnecessary impurities in a space to a harmless degree of cleanliness or to remove particles, volatile harmful substances, bacteria and other pollutants in the space within a certain space.

Air sterilization method: The most common method is to use steam to sterilize empty tanks. The conventional air sterilization method uses steam to sterilize the tank. The operations include turning on steam, exhausting air, maintaining pressure, draining water, turning on sterile air, etc., and are generally manual operations.


Fermentation tank undergoing routine maintenance

Advantages of steam air sterilization

High steam temperature and strong penetrating power

The latent heat of steam is large and can quickly increase the temperature of objects. The penetrating power of steam molecules is strong and can easily coagulate and denature proteins. Research shows that at high temperatures of 120°C-130°C, all bacterial spores can be killed in 20 to 30 minutes. , the sterilization pass rate reaches 100%.

Steam sterilization, no pollution and no residue

Steam is generated by a steam generator by heating water without adding any chemical components, ensuring the safety of sterilization from the source. In addition, no physical pollution will be produced during the sterilization process, and it can be truly pollution-free and residue-free. After the sterilization is completed, no waste gas or waste water will be produced, eliminating the trouble of discharge.

Steam sterilization time is short and efficient

In high-pressure steam sterilization, the sterilization temperature increases with the increase of steam pressure, which can greatly shorten the sterilization time and improve work efficiency. Saturated steam flows dynamically in the sterilization pot, and the heat energy of the steam is evenly distributed on the medical equipment. Under the action of high temperature and high pressure, bacteria are quickly killed.


The fermentation tank is running smoothly

research shows. When the steam temperature reaches above 132°C and is maintained for 10 minutes, all microorganisms including spores and spores with strong resistance can be killed.

Advantages of hot water air sterilization

Sterilization is faster and more thorough

When the air in the tank is removed through the temperature gradient of each heat transfer layer, using hot water is faster and more thorough than using steam. In the air elimination operation, exhaust is a very critical operation.

In the presence of less than 1mm of air, the steam is transmitted from 121°C to the place separated by air, and drops to just over 100°C. It is conceivable that at this temperature, if it is maintained for 30 minutes, the sterilization operation will not be able to reach the desired level. to the requirements. In hot water air exhaust, due to the obvious difference in specific gravity, hot water exhaust is faster and more thorough than steam exhaust.


Fermentation tank automatic sterilization tank cover is being debugged

Sterilization process is more energy efficient

The heat loss of hot water air sterilization is only the radiant heat conducted to the outside world, while the steam air sterilization process, exhaust and drainage all take away a lot of energy.

Sterilization results are highly reliable, consistent and highly automated

This is the same as CIP fully automatic cleaning technology. The use of hot water air disinfection is more conducive to the logical judgment of the automated system. Hot water air sterilization reduces the labor intensity of personnel, ensures the exhaust effect, and ensures no dead ends in sterilization. These ensure that sterilization is highly reliable and consistent.640-(5).jpg

The two-level automatic sterilization fermentation tank is running smoothly

At present, in the hygiene fields such as pharmaceuticals and food, air disinfection systems and cleaning-in-place systems are basically integrated. Only the combination of SIP system and CIP system can ensure the best cleaning and sterilization effect.