Protein separation and purification is a method that uses downstream bioengineering technology to separate and purify the desired target protein from a mixture.
There are many types of valves, each with its own advantages and disadvantages. Here are the advantages and disadvantages of the five major valves, including gate valves, butterfly valves, ball valves, globe valves and plug valves. I hope it will be helpful to you.
Alpha-arbutin, an alpha-glucoside extracted from hydrobenzoquinone, blocks melanin synthesis by effectively inhibiting the tyrosinase activity of melanocytes and is known for its major whitening properties. Furthermore, α-arbutin exhibits high antioxidant, antimicrobial, and anti-inflammatory activities and is widely used in the pharmaceutical and cosmetic industries.
In the biological reaction production process, the DO value is generally used as the dissolved oxygen parameter. The DO value is one of the most critical parameters in the entire fermentation process. It can not only intuitively reflect the dynamic changes of the entire fermentation process, but also indirectly reduce fermentation production time and energy consumption by increasing the DO value, and improve the yield and quality of the final product.
The rapid development of animal cell culture technology has promoted the development of production in the field of biopharmaceuticals, and large-scale animal cell culture technology has attracted much attention.
There are three main types of fermentation culture methods, namely batch culture, fed-batch culture and continuous culture.
After completing the cleaning of the fermentation system, the next step is to empty the fermentation tank (empty tank disinfection).
Chixian sugar alcohol is currently the only sugar alcohol sweetener produced by microbial fermentation in the world.
Fermentation broth pretreatment is an important step in extracting biochemical substances from microbial fermentation broth. Its purpose is not only to separate cells, bacteria and other suspended particles, but also to remove some soluble impurities and change the properties of the filtrate to facilitate subsequent operations.
How to find out whether the fermentation process is contaminated with bacteria? The cause of infection? The route of infection?Currently, the commonly used inspection methods in production include: ①microscopic inspection; ②plate streak inspection; ③broth culture inspection.Determining whether fermentation is contaminated with bacteria should be based on the results of the sterility test.
With the development of genetic engineering, a variety of highly effective drugs, sensitive diagnostic reagents and biotechnology products have been produced through the culture of animal cells. Currently, it is developing into a high-tech industry in this direction.
Centrifuges are widely used in daily life as well as in scientific and medical research to separate cells, subcellular organelles, viruses, proteins and nucleic acids.
Microalgae can effectively use light energy, carbon dioxide and inorganic salts to synthesize proteins, fats and a variety of high value-added bioactive substances. Microalgae can be cultivated to produce healthy food, food additives, animal feed, biofertilizers and other natural products.
Enzymes are biological catalysts produced by organisms for their own metabolic activities.The equipment required for biological reactions using enzymes as catalysts is called an enzyme reactor.It is an important part of current enzyme engineering research. Through the study of enzyme reactor kinetics and thermodynamic models, it can provide important theoretical basis for the design, selection and application of enzyme reactors.
Airlift fermenters are the most widely used bioreactors. The airlift reactor is developed on the basis of the bubble tower reactor. It uses the injection function of air and the difference in fluid weight to cause the circulating flow of the reaction liquid to achieve stirring, mixing and oxygen transfer of the liquid. That is, there is no need for mechanical stirring, and it relies entirely on the lifting of the gas to cause circulation and turbulence of the liquid, thereby achieving the purpose of mixing and transferring gas and liquid.
Depending on how the energy required by the reactor is input, microbial cell reactors can be classified as: mechanical, which inputs energy through mechanical agitation, aerobiological, which utilises the kinetic energy of a gas jet, and bioreactors, which circulate the liquid by using the jetting action of a pump on the liquid.
The transfer phenomena and processes of bioreactors are controlled by two mechanisms: convection and diffusion. In bioreactors, processes related to flow and diffusion are: agitation shear, mixing, dissolved oxygen mass transfer, heat transfer, and apparent kinetics. For microbial reaction systems, due to the characteristics of biological cells such as growth, adaptation, delay, degradation, mutation, and sensitivity to shear, bioreactors are more complex than ordinary chemical reactors, and their amplification process is more difficult.
The laboratory small fermentation tank is a laboratory desktop fermentation tank with flexible configuration, easy operation, and extremely small floor space. It adopts a full computer touch screen control system, color touch screen display, powerful expansion functions, and supports up to 24 fermentation parameters. Control is an experimental platform for research on fermentation process conditions and precision culture.
Fermentor performance depends on some key parameters, such as sterile environment, temperature, agitation, pH value, pressure, DO value (dissolved oxygen), etc. During the culture run, these parameters must be strictly monitored to allow the bioreactor to operate continuously under optimal conditions.
As the market scale in the field of biological fermentation continues to expand, fermentation tanks are widely used in bioengineering, pharmaceutical and other fields. Fermentation tank manufacturing technology has also progressed day by day, entering the stage of automation development, meeting various fermentation needs and improving fermentation quality.
In the fermentation tank and its ancillary equipment (air purification system, temperature, pressure flow and other control systems and corresponding pipeline valves), problems in any link may cause fermentation failure. Therefore, enterprises should first establish the concept of "prevention first" and start with equipment to eliminate various hidden dangers that may cause fermentation contamination.
In order to ensure the normal operation of the fermentation tank, users must not only be familiar with the working principle of the equipment, the structure and performance of each component, and the functions of pipelines and valves, but also pay attention to and strengthen the maintenance and upkeep of the equipment. Only by carefully maintaining the equipment can the Ensure that the equipment is in the best condition and extend the working life of the equipment.
Solid fermenter is a commonly used fermentation equipment in microbial culture. It has the advantages of compact structure, small footprint, simple and convenient operation, etc.
Electrode has the important role in fermentation bioreactor, such as Temperature electrode, PH electrode, DO probe etc. In this article, let’s talk about how to maintenance PH electrode.
The operation of the fermenter is a rigorous work, and how to control the parameters in the fermenter process is very important. There are many process parameters. Today we will talk about the tank temperature control and PH value control in the fermentation tank process.
Airlift fermenters are widely used biological reaction equipment. This type of reactor has the advantages of simple structure, not easy to contaminate bacteria, high efficiency of dissolved oxygen, and low energy consumption.
Glass Bioreactor are often used in microbiology laboratories of scientific research institutes, colleges and universities and enterprises. They are suitable for screening of microbial fermentation medium formulations, optimization of fermentation process parameters, and verification of production processes and strains. They are ideal test equipment for precision fermentation. .
The cleaning of the fermenter is very important. Different types of fermentation tanks have similar cleaning methods. We introduce the general cleaning method.
Fermenter is a kind of bioreactor, which refers to a device system that provides a suitable reaction environment for living cells or enzymes, allowing them to carry out cell proliferation or production. It also provides a suitable growth environment for the growth and reproduction of bacteria, and promotes the production of the products that people need. Fermenter can be divided into different types according to different classification methods. Let's first understand the different types of fermenters.
Airlift fermenters are also the most widely used biological reaction equipment. This type of reactor has the advantages of simple structure, not easy to contaminate bacteria, high efficiency of dissolved oxygen, and low energy consumption.
Fermenter are widely used in dairy products, beverages, bioengineering, pharmaceuticals, fine chemicals and other industries. The tank body and the upper and lower filling heads (or cones) are processed by spinning R angle. The inner wall of the tank is mirror-polished, and there is no hygienic dead angle. The fully enclosed design ensures that the materials are always mixed and fermented in a pollution-free state. The equipment is equipped with Air breathing holes, CIP cleaning nozzles, manholes and other devices.
Bioreactor refers to any manufacturing or engineering equipment that provides a biologically active environment, and uses the biological functions of enzymes or organisms to carry out biochemical reactions in vitro. It is a biological function simulator, such as a fermenter, a fixed enzyme or immobilized cell reactor, etc.